ABSTRACT
Accurate COVID-19 screening via molecular technologies is still hampered by bulky instrumentation, complicated procedure, high cost, lengthy testing time, and the need for specialized personnel. Herein, we develop point-of-care upconversion luminescence diagnostics (PULD), and a streamlined smartphone-based portable platform facilitated by a ready-to-use assay for rapid SARS-CoV-2 nucleocapsid (N) gene testing. With the complementary oligo-modified upconversion nanoprobes and gold nanoprobes specifically hybridized with the target N gene, the luminescence resonance energy transfer effect leads to a quenching of fluorescence intensity that can be detected by the easy-to-use diagnostic system. A remarkable detection limit of 11.46 fM is achieved in this diagnostic platform without the need of target amplification, demonstrating high sensitivity and signal-to-noise ratio of the assay. The capability of the developed PULD is further assessed by probing 9 RT-qPCR-validated SARS-CoV-2 variant clinical samples (B.1.1.529/Omicron) within 20 mins, producing reliable diagnostic results consistent with those obtained from a standard fluorescence spectrometer. Importantly, PULD is capable of identifying the positive COVID-19 samples with superior sensitivity and specificity, making it a promising front-line tool for rapid, high-throughput screening and infection control of COVID-19 or other infectious diseases.
ABSTRACT
Against the background of the outbreak of coronavirus disease 2019 (COVID-19) . in order to further understand and clarify the current global status and hotspots of the COVID-19, this paper is based on the Web of Science database to search for this topic. As of April 30, 2020, the SCI literature in the field of SARS-CoV-2 will be subjected to a metrological analysis to discuss the research focus and research status of SARS-CoV-2. This research analyzed the research situation of this research field through the information acquisition, processing and analysis of SARS - CoV - 2 related literature, and the application of bibliometrics methods. Statistical analysis data and visual data analysis results showed that a total of 1, 523 documents had been searched with "COVID - 19" as the subject term;at the current stage of research, China ranked first in the number of articles published in this field;University of London, Harvard University and Huazhong University of Science and Technology were among the top three institutions in the world in terms of publication volume;sorting out high-frequency key words to identify the research hotspots as COVID-19, SARS and public health. It is found that Chinese scholars have achieved rapid development in the field of "COV ID-19", and they are at the forefront of the world in terms of the influence of articles and the amount of articles published.
ABSTRACT
Background: Little research has been done on the mental health of western Chinese university students during the Coronavirus disease (COVID-19) pandemic. This work aims to fill the gap by gaining insight into the positive psychological capital and its influencing factors among university students in western China during the COVID-19 pandemic. Methods: An online cross-sectional study was conducted in college students from a medical school. Participants were surveyed anonymously using a general information questionnaire and the Positive Psychological Capital Scale. The key factors and models affecting the psychological capital were obtained by using the gradual regression method. If P < 0.05, the difference would be considered statistically significant. Results: The total score of positive psychological capital among university students in western China was 118.51±16.91, Taking the psychological capital as the dependent variable, gender and grade as the independent variables, the key factors and models affecting the psychological capital were obtained by using the gradual regression method.The results showed that the P-value of each influencing factor was <0.05. The key influencing factors of psychological capital of college students were gender and and place of residence. Conclusion: The overall positive psychological capital of college students in western China was in good condition during the pandemic, with large room for improvement though. Gender and Residence were independent influencing factors on the positive psychological capital.